Eucidating the ultrastructure of enterovirus Replication Organelles

We will delve into the intricacies of enterovirus replication by harnessing the cutting-edge technique of cryo-electron tomography (cryo-ET). Enteroviruses (e.g. poliovirus, coxsackievirus, rhinovirus) orchestrate extensive architectural transformations within host cells during infection, a process essential for the creation of specialized replication organelles (ROs) responsible for replication of the viral RNA genome and generating progeny virions. Recent advancements in the field have made it possible to explore the ultrastructure of ROs, and my research will capitalize on these breakthroughs.

Primary objective will be to shed light on the contributions of viral proteins and host factors to the ultrastructure and formation of ROs. Cryo-ET offers a unique advantage by allowing us to directly visualize these organelles within their cellular context. This approach may yield ground-breaking insights into the roles played by enteroviral proteins and host factors throughout the infection process, significantly expanding our understanding of these important human pathogens.

Bob Coelingh