Determining the structure and components of the enterovirus Replication Complex and Replication Organelle

The goal of this research project is to elucidate the spatial and temporal regulation of the life cycle of enteroviruses (e.g. poliovirus, coxsackievirus, rhinovirus). Some enteroviral proteins are reported to interact with host factors, thereby forming platforms conducive to the replication of the viral genome, which are referred to as replication complexes (RCs). By interacting with host factors, these viral proteins are also intricately involved in remodelling cellular membranes, leading to the creation of membranous structures where viral replication occurs, known as replication organelles (ROs).

Primary focus will be on understanding the structure and identifying the viral and cellular components integral to enterovirus RCs and ROs. To achieve this objective, we will employ diverse techniques, including biochemistry, cryo-electron microscopy, proteomics, and lipidomics. Additionally, we intend to provide further insights into the roles played by select enteroviral proteins through the utilization of cutting-edge fluorescent microscopy technologies.

Collectively, these findings will enhance our understanding of enterovirus replication and subsequently facilitate the development of more rational strategies for designing antiviral compounds.

Víctor Pisabarro Montoro